熒光適配器用于斑馬魚代謝疾病研究

體視顯微鏡熒光適配器用于mCherry和eGFP在斑馬魚上的表達

馬里蘭大學(xué)巴爾的摩分校使用美國NIGHTSEA的SFA體視顯微鏡熒光適配器系統(tǒng)用于篩選他們的轉(zhuǎn)基因斑馬魚。該研究小組研究了與代謝性疾病相關(guān)的基因的功能，包括糖尿病，肥胖癥和NAFLD / NASH（非酒精性脂肪性肝病/非酒精性脂肪性肝炎）。產(chǎn)生胰島素的β（β）細胞發(fā)生在胰腺的胰島中，在維持葡萄糖水平方面至關(guān)重要。它們是代謝疾病社區(qū)的一個重要研究課題，斑馬魚β細胞在功能和發(fā)育方面都與哺乳動物相似。在我們觀察的幼蟲發(fā)育階段，Dunleavey博士估計胰島含有大約35 β細胞。斑馬魚模型表達了兩種熒光報告基因—— 胰腺外分泌的eGFP和β細胞中的mCherry（ptf1a：eGFP和ins：mCherry，也稱為exocrine eGFP和β細胞mCherry）——Dunleavey博士希望能夠同時看到它們。

下面的圖像是用iPhone手持在配備美國nightsea SFA的尼康SMZ1000體視顯微鏡的目鏡上快速制作的。SFA-RB熒光適配器皇家藍激發(fā)/發(fā)射組用于成像eGFP，SFA-GR熒光適配器綠色組用于成像mCherry。上海峰志儀器有限公司代理銷售美國nightsea公司生產(chǎn)的熒光適配器。

斑馬魚在白光照射下的圖像

斑馬魚在SFA-RB皇家藍激發(fā)下eGFP發(fā)出綠色熒光

斑馬魚在SFA-GR熒光適配器綠色激發(fā)下mCherry發(fā)出的紅色熒光

以下為引用的英文原文：

Zebrafish Metabolic Disease Reporters – mCherry and eGFP

NIGHTSEA recently visited Dr. Jessica Dunleavey at the Zaghloul Lab at the University of Maryland Baltimore to determine whether our Model SFA Stereo Microscope Fluorescence Adapter system could be used to screen their transgenic zebrafish. The research group investigates the functions of genes associated with metabolic diseases including diabetes, obesity, and NAFLD/NASH (nonalcoholic fatty liver disease / nonalcoholic steatohepatitis). Insulin-producing β (beta) cells occur in islets in the pancreas  and are critically important in maintaining glucose levels. They are a significant study topic in the metabolic disease community, and zebrafish β cells are similar to those in mammals both functionally and developmentally. At the stage of larval development we looked at, Dr. Dunleavey estimated that the islet contained approximately 35 β cells. The zebrafish model expresses two fluorescent reporters – eGFP in the exocrine pancreas and mCherry in the β cells (ptf1a:eGFP and ins:mCherry, also known as exocrine eGFP and β cell mCherry) – and Dr. Dunleavey hoped to be able to see both of them.

The test was successful. The images below were made quickly with an iPhone hand-held over the eyepiece of a Nikon SMZ1000 stereo microscope outfitted with the NIGHTSEA SFA. The Royal Blue excitation/emission set was used for imaging eGFP, and the Green set for imaging mCherry.

上海峰志儀器有限公司代理銷售美國nightsea、美國路陽生產(chǎn)的體視顯微鏡熒光適配器和熒光手電筒。有關(guān)體視顯微鏡熒光適配器產(chǎn)品介紹請瀏覽《美國nightsea熒光適配器 》，上海峰志儀器有限公司銷售的熒光觀察設(shè)備能夠觀察植物愈傷、葉片、種子、根系等上面的轉(zhuǎn)基因的表達，還能觀察轉(zhuǎn)基因在動物如老鼠、斑馬魚、果蠅等實驗動物上的表達。有便攜式單波長熒光手電筒，也有手持式大面積照射高強度的雙波長激發(fā)光源，上海峰志提供免費試機，有興趣的老師和同學(xué)可按照網(wǎng)頁底部聯(lián)系聯(lián)系。