紫外交聯儀Stratalinker UV 2400
型號:ML29-Stratalinker UV 2400
紫外交聯儀Stratalinker UV 2400特點:
為了使核苷酸固定在膜上,傳統的方法是將膜置于真空烘箱中80度烘2小時,在紫外交聯儀上僅需30-60秒
與真空烘焙相比,大大增加雜交信號強度
內部配備光度檢測儀,可以補償由于紫外燈泡老化造成的輸出偏移
具自動交聯、時間和能量三種模式供選擇
帶有顯示時間、能量的顯示器及設置面板
紫外交聯儀Stratalinker UV 2400應用:
將核酸共價結合到固相支持物(如硝酸纖維素膜)上
Northem,Southern,dot及slot印跡分析
雜交芯片的制備
菌落、噬菌斑篩選,瓊脂糖凝膠中DNA的切割
產生胸腺二聚體,用于部分限制性內切酶消化作圖
紫外敏感性測試,用于鑒定recA宿主菌
樣品紫外照射處理
誘導培養細胞的凋亡
照射波長:254nm(配8瓦254nm紫外線燈管5根)
內部尺寸:47.7W/34.3D/16.8H
紫外交聯儀Stratalinker UV 2400的特點:
用于將 DNA 或 RNA 交聯到涂層載玻片、尼龍、硝化纖維素或尼龍增強硝化纖維素膜上
將寶貴的時間從幾個烤箱烘烤小時減少到30-60秒
與烤箱烘烤相比,增加雜交信號
內部光電探測器補償老化UV燈泡的偏移輸出
可在自動交叉、時間或能量模式下操作
安捷倫基因組學/Stratagene UV 交聯劑設計用于將 DNA 或 RNA 交聯到尼龍、硝酸纖維素或尼龍增強的硝化纖維素膜上。交聯過程僅需25-50秒,而傳統的方法是在80°C下烘烤過濾器2小時。此外,與烤箱烘烤相比,交聯已被證明可以顯著增加雜交信號。為了獲得佳交聯性能,每個Stratalinker紫外交聯劑都配備了一個內部光電探測器,旨在補償老化紫外線燈泡功率輸出的自然變化。
Stratalinker UV紫外交聯儀可用于Northern, Southern, dot or slot blot 分析,1-3菌落或斑塊篩選,在印跡之前在瓊脂糖凝膠中劃傷DNA,4二聚體形成以進行部分消化以進行快速限制性映射,5用于宿主應變驗證的UV靈敏度測試,6和PCR樣品的紫外線照射。 時間模式或能量模式,該設備有兩種方便的尺寸:用于較小膜的 Stratalinker 1800 UV紫外交聯儀和用于較大膜的 Stratalinker 2400 UV紫外交聯儀。
紫外交聯儀Stratalinker UV 2400英文介紹:
Features:
For Crosslinking DNA or RNA to Coated Glass Slides, Nylon, Nitrocellulose or Nylon-Reinforced Nitrocellulose Membranes
Greatly Reduces Valuable Time From Several Oven Baking Hours to 30-60 Seconds
Increases Hybridization Signal Compared to Oven Baking
Internal Photodetector Compensates for Shifting Output of Aging UV Bulbs
Can be Operated in Autocrosslink, Time or Energy Modes
The Agilent Genomics / Stratagene UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25-50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crosslinking performance, each Stratalinker UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.
The Stratalinker UV crosslinker may be used for Northern, Southern, dot or slot blot analysis,1-3 colony or plaque screening, nicking of DNA in agarose gels prior to blotting, 4 dimer formation to perform partial digests for rapid restriction mapping,5 UV sensitivity testing for host strain verification,6 and UV irradiation of PCR samples.7 The Stratalinker UV crosslinker may be used in the Autocrosslink, Time, or Energy modes, and the apparatus is available in two convenient sizes-the Stratalinker 1800 UV crosslinker for smaller membranes and the Stratalinker 2400 UV crosslinker for larger membranes.